Background: Mesenchymal stem cells (MSCs) have been widely proven effective for therapeutic angiogenesis in\nischemia animal models as well as clinical vascular diseases. Because of the invasive method, limited resources,\nand aging problems of adult tissue-derived MSCs, more perinatal tissue-derived MSCs have been isolated and\nstudied as promising substitutable MSCs for cell transplantation. However, fewer studies have comparatively\nstudied the angiogenic efficacy of MSCs derived from different tissues sources. Here, we evaluated whether the\nin-situ environment would affect the angiogenic potential of MSCs.\nMethods: We harvested MSCs from adult bone marrow (BMSCs), adipose tissue (AMSCs), perinatal umbilical cord\n(UMSCs), and placental chorionic villi (PMSCs), and studied their ââ?¬Å?MSC identityââ?¬Â by flow cytometry and in-vitro\ntrilineage differentiation assay. Then we comparatively studied their endothelial differentiation capabilities and\nparacrine actions side by side in vitro.\nResults: Our data showed that UMSCs and PMSCs fitted well with the minimum standard of MSCs as well as\nBMSCs and AMSCs. Interestingly, we found that MSCs regardless of their tissue origins could develop similar\nendothelial-relevant functions in vitro, including producing eNOS and uptaking ac-LDL during endothelial\ndifferentiation in spite of their feeble expression of endothelial-related genes and proteins. Additionally, we\nsurprisingly found that BMSCs and PMSCs could directly form tubular structures in vitro on Matrigel and their\nconditioned medium showed significant proangiogenic bioactivities on endothelial cells in vitro compared\nwith those of AMSCs and UMSCs. Besides, several angiogenic genes were upregulated in BMSCs and PMSCs\nin comparison with AMSCs and UMSCs. Moreover, enzyme-linked immunosorbent assay further confirmed that\nBMSCs secreted much more VEGF, and PMSCs secreted much more HGF and PGE2.\nConclusions: Our study demonstrated the heterogeneous proangiogenic properties of MSCs derived from\ndifferent tissue origins, and the in vivo isolated environment might contribute to these differences. Our study\nsuggested that MSCs derived from bone marrow and placental chorionic villi might be preferred in clinical\napplication for therapeutic angiogenesis.
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